CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

Specifications

Product Data
Clone Name	YTH34.5
Applications	CT, ELISA, FC, IHC, IP, WB
Recommend Dilution	ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays: 50 µg/ml. Use human serum as complement source. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 1 x 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Resin Sections.  This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (Ref.2). Recommended Positive Control: Tonsil.
Reactivity	Human, Monkey
Host	Rat
Clonality	Monoclonal
Immunogen	Human lymphocytes.
Specificity	SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
Formulation	PBS State: Low Endotoxin State: Liquid purified IgG fraction from tissue culture supernatant Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD52 molecule
Database Link	Entrez Gene 1043 Human UniProt ID P31358
Background	The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyms	HE5, CDw52
Reference Data

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