CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

Specifications

Product Data
Clone Name	YTH34.5
Applications	CT, ELISA, FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/20 diluted antibody to label 1 x 10e6 cells in 100ul. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on Paraffin Sections: 1/20-1/40. Immunohistochemistry on Resin. Positive Control Tissue: Tonsil. ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays: 50 µg/ml. Use Human serum as complement source.
Reactivity	Human, Monkey
Host	Rat
Clonality	Monoclonal
Immunogen	Human lymphocytes.
Specificity	This antibody reacts with CD52 antigen. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD52 molecule
Database Link	Entrez Gene 1043 Human UniProt ID P31358
Background	CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyms	HE5, CDw52
Reference Data

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