Il2ra Mouse Monoclonal Antibody [Clone ID: OX-39]

Specifications

Product Data
Clone Name	OX-39
Applications	ELISA, FC, IHC, IP
Recommend Dilution	Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. ELISA. Immunoprecipitation. Immunohistochemistry on frozen and paraffin sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.
Specificity	This antibody recognizes the alpha chain of CD25, otherwise known as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. We recommend the use of SM265LE for this purpose.
Formulation	PBS, pH7.4 containing 0.02% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 25704 Rat UniProt ID P26897
Background	CD25 is a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Reference Data

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