Ptprc (CD45RA) Mouse Monoclonal Antibody [Clone ID: OX-33]

Specifications

Product Data
Clone Name	OX-33
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine- paraformaldehyde) - refer to ref. Whiteland, J.L. et al. (1995) - reference 4.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified spleen leucocyte common antigen. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line.
Specificity	This antibody is directed against a high molecular weight band of the leucocyte common antigen (LCA). MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 24699 Rat UniProt ID P04157
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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