HIV-1 + HIV-2 Protease Mouse Monoclonal Antibody [Clone ID: 1696]

Specifications

Product Data
Clone Name	1696
Applications	ELISA, FN, WB
Recommend Dilution	ELISA. Western blotting: 0.5 μg/ml (Reducing conditions).  Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Functional Assay: Antibody SM3051A strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases.
Reactivity	Human Immunodeficiency Virus 1, Human Immunodeficiency Virus 2
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed full-length HIV-1 Protease
Specificity	The antibody recognizes free N-terminus of mature HIV protease HIV1 and HIV2, but it does not react with the precursor.
Formulation	Azide Free Phosphate Buffered saline (PBS), pH~7.4, 0.2 μm filter sterilized State: Azide Free State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents.
Synonyms	HIV1, HIV-I, Human immunodeficiency virus type 1
Reference Data

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