LCK Mouse Monoclonal Antibody [Clone ID: LCK-01]

Specifications

Product Data
Clone Name	LCK-01
Applications	FC, IF, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Western Blotting. Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide corresponding to amino acids 22-36 in the sequence of Human Lck
Specificity	The antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase. Negative Species: Mouse.
Formulation	Phosphate Buffered Saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Affinity Chromatography on Protein A.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	LCK proto-oncogene, Src family tyrosine kinase
Database Link	Entrez Gene 3932 Human UniProt ID P06239
Background	Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its z subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Synonyms	p56-LCK, LSK
Reference Data

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