alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: AFP-11]

Specifications

Product Data
Clone Name	AFP-11
Applications	ELISA, IHC, R, WB
Recommend Dilution	Western Blot: 2 μg/ml, non-reducing conditions. Immunohistochemistry on paraffin sections: 10 µg/ml; Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB. Positive control: hepatocellular carcinoma. ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody clone AFP-01 (cat. no. SM3089P/PX). RadioImmunoAssay.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified alpha-Fetoprotein
Specificity	The antibody detects Alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa.
Formulation	PBS, pH 7.4 containing 15 mM sodium azide as preservative State: Purified State: Liquid purified immunoglobulin fraction
Concentration	lot specific
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alpha fetoprotein
Database Link	Entrez Gene 174 Human UniProt ID P02771
Background	AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Synonyms	Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
Reference Data

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