PAG (PAG1) Mouse Monoclonal Antibody [Clone ID: PAG-C1]

Specifications

Product Data
Clone Name	PAG-C1
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Positive control: RAJI human Burkitt lymphoma cell line. Immunohistochemisry on paraffin sections: heat-mediated antigen retrieval in citrate buffer pH 6.1. Positive tissue: Appendix (germinal center of lymphatic follicle.
Reactivity	Human, Mouse, Rat, Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH
Specificity	The antibody recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography, purity > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Database Link	Entrez Gene 55824 Human UniProt ID Q9NWQ8
Background	PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Synonyms	PAG, CBP
Reference Data

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