beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-02]

Specifications

Product Data
Clone Name	B2M-02
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval; recommended dilution: 5 μg/ml; positive tissue: liver. Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified isolated human beta2-microglobulin
Specificity	The antibody B2M-02 reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin.
Formulation	PBS with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	beta-2-microglobulin
Database Link	Entrez Gene 567 Human UniProt ID P61769
Background	B2M is a 12 kDa Ig like glycoprotein. B2M non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms	B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data

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