CD45 / LCA Mouse Monoclonal Antibody [Clone ID: K252-1E4]

Specifications

Product Data
Clone Name	K252-1E4
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 106 cells in 100 µl.  Immunofluorescence. Immunohistochemistry on Frozen Sections.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine Peripheral Blood Lymphocytes.  Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Specificity	All Porcine leucocytes. This K252.1E4 antibody recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998). CD45 is also known as leukocyte common antigen (LCA). K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermann et al. 1994).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Afinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q6SZ85
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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