IKB alpha (NFKBIA) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, EMSA, IP, WB
Recommend Dilution	ELISA: 1:2000. Western Blot: 1:200 - 1:1000. Immunoprecipitation. Gel Super Shift Assays.
Reactivity	Human
Host	Sheep
Clonality	Polyclonal
Immunogen	Human IkB alpha expressed in E. coli
Specificity	This antibody recognises IkB alpha, and identifies a single polypeptide band of 39kD in Western blots.
Formulation	PBS containing 0.09% Sodium Azide and 0.5% Bovine Serum Albumin State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	NFKB inhibitor alpha
Database Link	Entrez Gene 4792 Human UniProt ID P25963
Background	Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Synonyms	I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha
Reference Data

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