GAPDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: 0.5 - 1 µg/ml, detects a band of approximately 37 kDa in HeLa cell lysates. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 16 amino acid peptide located near human GAPDH carboxy-terminus
Specificity	This antibody recognises an epitope within the C-terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0,02% Sodium azide
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	Entrez Gene 2597 Human UniProt ID P04406
Background	GAPDH is a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes. GAPDH is a homotetramer molecule consisting of four 36 kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate. The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimer's disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdon's disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (1).
Synonyms	GAPD, CDABP0047
Reference Data

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