WNT3 Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, PEP-ELISA, WB
Recommend Dilution	ELISA: 1:16,000; WB: 1-3 ug/ml; IHC: 2-4 ug/ml
Reactivity	Human, Mouse, Rat, Dog
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	43271 Da
Gene Name	Wnt family member 3
Database Link	NP_110380 Entrez Gene 22415 MouseEntrez Gene 24882 RatEntrez Gene 609107 DogEntrez Gene 7473 Human UniProt ID P56703
Background	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq]
Synonyms	INT4; TETAMS
Note	In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway

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