ADO Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1000-5000 WB positive control: Mouse testis tissue IHC: 25-100 Positive control: Human liver cancer Predicted cell location: Cytoplasm
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a region derived from 161-174 amino acids of Human 2-aminoethanethiol (cysteamine) dioxygenase
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	30 kDa
Gene Name	2-aminoethanethiol (cysteamine) dioxygenase
Database Link	NP_116193 Entrez Gene 211488 MouseEntrez Gene 84890 Human UniProt ID Q96SZ5
Background	Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine; whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference; hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Synonyms	C10orf22
Reference Data
Protein Pathways	Metabolic pathways, Taurine and hypotaurine metabolism

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