CD3E Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	WB 1:500 - 1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human CD3E
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CD3e molecule
Database Link	NP_000724 Entrez Gene 12501 MouseEntrez Gene 315609 RatEntrez Gene 916 Human UniProt ID P07766
Background	When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ¦Æ, ¦Ã, ¦Å and ¦Ä. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3¦Å, such that a proline-region is exposed and then associates with the adapter protein Nck (5).1. Kuhns, M.S. et al. (2006) Immunity 24, 133-139.2. Pitcher, L.A. and van Oers, N.S. (2003) Trends Immunol. 24, 554-560.3. Osman, N. et al. (1996) Eur. J. Immunol. 26, 1063-1068.4. Hatada, M.H. et al. (1995) Nature 377, 32-38.5. Gil, D. et al. (2002) Cell 109, 901-912.
Synonyms	IMD18; T3E; TCRE
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway

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