Cannabinoid Receptor II (CNR2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human Cannabinoid Receptor 2. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cannabinoid receptor 2
Database Link	NP_001832 Entrez Gene 57302 RatEntrez Gene 1269 Human UniProt ID P34972
Background	Cannabinoids have been used as pain relievers in eastern medicine for many years. To date, two specific cannabinoid receptors have been identified: the Cannabinoid Receptor 1 (CB1R) and the Cannabinoid Receptor 2 (CB2R). The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution. Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein. The CB2R is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas. CB2R expression in brain is still much less characterized than that of CB1R. Recently, it was demonstrated that CB2R is expressed in brain and might have role in controlling fundamental processes such as proliferation and survival of neural cells. Overexpression of CB2R was reported in several cancers such as prostate, glioma and acute myeloid leukemias.In human astrocytoma a direct relation between CB2R expression and tumor malignancy was demonstrated. Activation of CB2R, in vivo, by its agonist JWH-133, completely blocked cell growth. In C6 glioma it was shown that activation of the CB2R by JWH-133 resulted in internalization of only the CB2R, and not CB1R leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.
Synonyms	CB-2; CB2; CX5
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction

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