Chrm3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000; Immunocytochemistry: 1:400 (on rat primary astrocytes)
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat m3. 3rd intracellular loop.
Specificity	TA328871 is a highly specific antibody directed against an epitope of the rat M3 muscarinic receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize M3 from human, mouse, and rat samples.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cholinergic receptor, muscarinic 3
Database Link	NP_036659 Entrez Gene 1131 HumanEntrez Gene 12671 MouseEntrez Gene 24260 Rat UniProt ID P08483
Background	The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5. The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues. The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscle. Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control. They also participate in learning and memory processing. The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves. m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholine. m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues. Involvement of muscarinic receptors in apoptosis and cell growth has been reported. m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors. Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, unclear. In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.
Synonyms	HM3
Reference Data

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