Chrna3 Rabbit Polyclonal Antibody

CAT#: TA328883

Rabbit Polyclonal Anti-Nicotinic Acetylcholine Receptor alpha3 (extracellular)



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CNY 11,000.00


货期*
7周

规格
    • 50 ul

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Specifications

Product Data
Applications IHC, WB
Recommend Dilution WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Peptide (C)KWKPSDYQGVEFMR, corresponding to amino acid residues 91-104 of rat nAChRa3. Extracellular, N-terminus.
Formulation Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification Affinity purified on immobilized antigen.
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Gene Name cholinergic receptor nicotinic alpha 3 subunit
Background Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore.All a subunits are expressed in neuronal cells except for the a1 subunit which is specifically expressed in the muscle. In the peripheral autonomic nervous system, nicotinic receptors are prominent and are responsible mediating fast synaptic transmission in all peripheral autonomic ganglia. In this system, these channel receptors are mostly made up of two a3 subunits heteromerized with three other subunits. The a3 subunit plays a most important role in this system as knockout mice suffer from severe autonomic failure accompanied by gastrointestinal and bladder malfunctions.Autoimmune autonomic ganglionopathy (AAG) is a neurological disorder characterized by dysfunctions in sympathetic, parasympathetic and enteric functions. Patients suffering from the disease express high levels of self antibodies against ionotropic nicotinic receptors, namely a3 subunits4. The a3 subunit is also found in dorsal root ganglion where it is most probably involved in nociception.
Synonyms LNCR2; MGC104879; NACHRA3; PAOD2
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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