Aryl hydrocarbon Receptor (AHR) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ICC/IF, IHC, WB
Recommend Dilution	Chromatin Immunoprecipitation, Western Blot, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry: 1:100 - 1:200, Immunohistochemistry-Paraffin: 1:100 - 1:200
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	N-terminal sequence of Aryl hydrocarbon Receptor purified from C57BL/6J mice. [UniProt# P30561]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	aryl hydrocarbon receptor
Database Link	NP_001612 Entrez Gene 11622 MouseEntrez Gene 25690 RatEntrez Gene 196 Human UniProt ID P35869
Background	Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR- mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.
Synonyms	bHLHe76
Note	This Aryl hydrocarbon Receptor antibody is useful for Gel Super Shift Assays. Suggested working dilution: add 4 ul Aryl hydrocarbon Receptor antibody to a 15 ul final volume of EMSA sample. This antibody does not work in Western blot. Use in Chromatin Immunoprecipitation reported in the scientific literature (PMID: 14718646). Use in Immunofluorescence reported in the scientific literature (PMID: 18515748). Use in Electron Microscopy and western blot reported in scientific literature (PMID 23999131) Other applications have not been tested.
Reference Data
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

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