Calnexin (CANX) Rabbit Polyclonal Antibody

CAT#: TA336279

Rabbit Polyclonal Calnexin Antibody



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CNY 5,381.00


货期*
5周

规格
    • 100 ul

Cited in 1 publication.
经常一起买 (4)
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Recombinant protein of human calnexin (CANX), transcript variant 1, 20 µg
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Recombinant protein of human calnexin (CANX), transcript variant 1, 100 µg
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Specifications

Product Data
Applications FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution Flow Cytometry, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:50, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunoprecipitation: 1:100
Reactivity Human, Mouse, Rat, Hamster
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to a C-terminal portion of the rat Calnexin protein (between residues 550-591) [UniProt P35565]
Formulation PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 97 kDa
Gene Name calnexin
Background Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.
Synonyms CNX; IP90; P90
Note This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, endoplasmic reticulum staining was observed in HeLa cells. In Western Blot, a band is seen ~90 kDa representing Calnexin. In IHC-P, staining was observed in the endoplasmic reticulum of mouse bladder tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families Druggable Genome, Transmembrane
Protein Pathways Antigen processing and presentation
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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