NG2 (CSPG4) Mouse Monoclonal Antibody [Clone ID: LHM 2]

Specifications

Product Data
Clone Name	LHM 2
Applications	FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow (Cell Surface): 1 - 2.5 ug/mL, Immunoprecipitation: 1:10 - 1:100, Flow Cytometry: 1 ug/ml, Knockout Validated, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry-Frozen: 1:200
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A 375P cells crude extract
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	chondroitin sulfate proteoglycan 4
Database Link	NP_001888 Entrez Gene 1464 Human UniProt ID Q6UVK1
Background	NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.
Synonyms	HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
Note	This NG2 Antibody (LHM 2) is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on both frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at ~300 kDa.
Reference Data
Protein Families	Transmembrane

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