Caspase 3 (CASP3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution	Flow (Intracellular), Immunohistochemistry-Frozen, Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin: 1:1000-1:5000, Immunoprecipitation: 1:50-1:200, Immunohistochemistry
Reactivity	Human, Mouse, Rat, Canine, Gerbil
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant catalytically active human caspase-3 protein was used as immunogen.
Formulation	Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	caspase 3
Database Link	NP_116786 Entrez Gene 12367 MouseEntrez Gene 25402 RatEntrez Gene 836 Human UniProt ID P42574
Background	Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p20-p10. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-3 polyclonal antisera recognizes the large (~14-21 kDa) and small (~10 kDa) subunits of active/cleaved caspase-3. Whereas the antisera has a strong preference for active/cleaved caspase-3, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-3 (~32 kDa).
Synonyms	CPP32; CPP32B; SCA-1
Note	Preferentially detects active caspase-3 (large subunit: ~14-21 kDa, and small subunit: ~10 kDa). However, it may also detect pro-caspase-3 (~32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3. Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis

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