Actin (ACTA1) Mouse Monoclonal Antibody [Clone ID: mAbGEa]

Specifications

Product Data
Clone Name	mAbGEa
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Western Blot: 1:100-1:1000, ELISA: 1:100 - 1:2000, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Simple Western: 1:25, Immunocytochemistry/ Immunofluorescence: 1:20-1:100, Flow Cytometry: 1 ug/ml, Flow (Intracellular): 1 ug/ml
Reactivity	Human, Mouse, Rat, Bovine, Chinese Hamster, Drosop
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46]
Formulation	Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	IgM purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	actin, alpha 1, skeletal muscle
Database Link	NP_001091 Entrez Gene 11459 MouseEntrez Gene 29437 RatEntrez Gene 58 Human UniProt ID P68133
Background	Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility.
Synonyms	ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM
Note	This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Stem cell - Pluripotency

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