Neurofilament (NEFL) Mouse Monoclonal Antibody [Clone ID: DA2]

Specifications

Product Data
Clone Name	DA2
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:250, Simple Western: 1:50, Immunohistochemistry-Paraffin, Western Blot: 1:5000 - 1:10000, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:500
Reactivity	Human, Mouse, Rat, Mammalian, Avian
Host	Mouse
Clonality	Monoclonal
Immunogen	Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	neurofilament, light polypeptide
Database Link	NP_006149 Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 4747 Human UniProt ID P07196
Background	Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.
Synonyms	CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110
Note	This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Amyotrophic lateral sclerosis (ALS)

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