GRP78 (HSPA5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:20, Simple Western: 1:25, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin, Immunohistochemistry
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654). [UniProt# P06761]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	78 kDa
Gene Name	heat shock protein family A (Hsp70) member 5
Database Link	NP_005338 Entrez Gene 14828 MouseEntrez Gene 25617 RatEntrez Gene 3309 Human UniProt ID P11021
Background	Within the ER misfolded proteins are detected by Bip(GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulated transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.
Synonyms	BIP; GRP78; HEL-S-89n; MIF2
Note	This GRP78 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot this antibody detects a 78 kDa protein representing GRP 78 from rat liver extract and HeLa cells. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Antigen processing and presentation, Prion diseases

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