TMS1 (PYCARD) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Flow (Intracellular): 1 - 2 ug/ml, Immunomicroscopy, Flow Cytometry: 1 - 2 ug/ml, Immunoprecipitation, Western Blot: 2.0 - 4.0 ug/ml, Simple Western: 1:1000, Immunohistochemistry: 1:400, Immunohistochemistry-Paraffin: 1:400, Immunocytochemistry/ Immunofluorescence: 1:40-1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal portion of the human ASC/TMS1 protein (between residues 1-50) [UniProt Q9ULZ3]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	PYD and CARD domain containing
Database Link	NP_037390 Entrez Gene 66824 MouseEntrez Gene 282817 RatEntrez Gene 29108 Human UniProt ID Q9ULZ3
Background	Target of methylation-induced silencing 1 protein, TMS1 (ASC, Pycard) is a 21 kDa cytoplasmic adaptor protein containing 2 protein-protein interaction domains: an N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). Both domains are members of the six-helix bundle death domain superfamily. These domains, via caspase activation, mediate the assembly of large signaling complexes that are involved in the apoptotic and inflammatory signaling pathways. During apoptosis TMS1 aggregates near the nuclear periphery as ball-like structures. Recent research suggests that it can modulate MAPK expression by suppressing the DUSP10/MKP5 phosphatase thus allowing the phosphorylation of MAPK by pathogens and toll-like receptor agonists. PMID: 2148701.
Synonyms	ASC; CARD5; TMS; TMS-1; TMS1
Note	This ASC/TMS1 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

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