SUV420h1 (KMT5B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:200, Western Blot (Negative), Flow Cytometry: 2-5 ug/ml
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human SUV420h1 protein (between residues 450-500) [UniProt Q4FZB7]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lysine methyltransferase 5B
Database Link	NP_060105 Entrez Gene 225888 MouseEntrez Gene 51111 Human UniProt ID Q4FZB7
Background	SUV420H1 (Histone-lysine N-methyltransferase 5B, KMT5B) is a histone methyltransferase that specifically di- and trimethlyates lysine 20 of H4 (H4 K20Me3), an epigenetic mark of transcriptional repression. This mark, along with trimethylation of histone 3 lysine 9 (H3 K9me3) and monomethylation of H3 lysine 27 (H3 K27me1), define genomic regions of constitutive heterochromatin that are poor in activated genes. This enzyme is essential for development, with Suv420h1 knockout mice proving lethal perinatally and showing overall growth retardation. The RB1 family of proteins have been shown to interact with, and target, KMT5B to histones, enabling the enzyme to function.
Synonyms	CGI-85; CGI85; SUV420H1
Note	This SUV420h1 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in A431 cells. In IHC-P, staining was observed in the nucleus of mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation

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