TLR5 Mouse Monoclonal Antibody [Clone ID: 85B152.5]

Specifications

Product Data
Clone Name	85B152.5
Applications	FC, IHC, WB
Recommend Dilution	Flow (Cell Surface), Immunohistochemistry-Paraffin, Flow (Intracellular), Immunohistochemistry, Flow Cytometry: 1-3 ug/10^6 cells in 100 ul, Western Blot: 1-3 ug/ml
Reactivity	Human, Mouse, Canine
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	100 kDa
Gene Name	toll like receptor 5
Database Link	NP_003259 Entrez Gene 53791 MouseEntrez Gene 7100 Human UniProt ID O60602
Background	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.
Synonyms	MELIOS; SLE1; SLEB1; TIL3
Note	Reported use in Flow Cytometry (cell surface): see Wong et al, 2007 for details.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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