Apolipoprotein E (APOE) Mouse Monoclonal Antibody [Clone ID: 1H4]

Specifications

Product Data
Clone Name	1H4
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Western Blot: 1:500 - 1:2000, ELISA: 1:10000, Immunohistochemistry, Immunohistochemistry-Paraffin: 1:200 - 1:1000, Flow Cytometry: 1:200 - 1:400
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	36 kDa
Gene Name	apolipoprotein E
Database Link	NP_000032 Entrez Gene 348 Human UniProt ID P02649
Background	Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Synonyms	AD2; APO-E; LDLCQ5; LPG
Note	This ApoE (1H4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
Protein Pathways	Alzheimer's disease

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.