RP1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:400, Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human RP1 protein (between residues 2000-2156) [UniProt P56715]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	240 kDa
Gene Name	retinitis pigmentosa 1 (autosomal dominant)
Database Link	NP_006260 Entrez Gene 19888 MouseEntrez Gene 6101 Human UniProt ID P56715
Background	Retinitis pigmentosa 1 (RP1), also known as oxygen-regulated protein 1, retinitis pigmentosa 1 protein, ORP1, and DCDC4A, regulates the stability and length of microtubule based axoneme of photoreceptors. RP1 is critical for the differentiation of photoreceptor cells, and RP1 also influences the photosensitivity and outer segment morphogenesis of rod photoreceptors. Similarly, RP1 controls the correct orientation and higher order stacking of the outer segment disks along the photoreceptor axoneme. RP1 is found in the cytoplasm and has only been reported in the retina. RP1 is subject to phosphorylation, and mutations in RP1 cause autosomal recessive and dominant retinitis pigmentosa. Both of these diseases lead to degeneration of the retinal photoreceptor cells resulting in night blindness, loss of peripheral visual field, and eventually loss of central vision (PMID: 10391211).
Synonyms	DCDC4A; ORP1
Note	This RP1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 240 kDa. In ICC/IF cytoplasmic staining was observed in Arpe-19 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome

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