HDAC5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ICC/IF, IHC, WB
Recommend Dilution	Chromatin Immunoprecipitation, Western Blot: 1.0 - 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:50 - 1:100, Immunohistochemistry: 1:50 - 1:100, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:200, Chromatin Immunoprecipitation (ChIP)
Reactivity	Human, Mouse, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	130 kDa
Gene Name	histone deacetylase 5
Database Link	NP_001015053 Entrez Gene 15184 MouseEntrez Gene 10014 Human UniProt ID Q9UQL6
Background	HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).
Synonyms	HD5; NY-CO-9
Note	This HDAC5 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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