Insulin (INS) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Insulin. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide A-chain: Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys- Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn; B-chain: Phe-Val-Asn-Gln- His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu- Glu-Arg-Gly-Phe-Phe-Tyr-Pro-Lys-Thr (Disulfide bonds between Cys7 and Cys7/Cys20 and Cys19), coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	insulin
Database Link	Entrez Gene 3630 Human UniProt ID P01308
Background	Insulin is a 51-amino acid peptide hormone produced by beta cells of the pancreatic islets. It is a combination of two peptide chains named A-chain (21-amino acids) and B-chain (30-amino acids), which are linked by two disulfide bonds. Insulin regulates the metabolism of carbohydrates by promoting the absorption of glucose from the blood into liver. It is also involved in the protein synthesis of skeletal muscle as well as fat tissue through the tyrosine kinase receptor pathway. This antibody was generated by immunization of guinea pigs with Insulin coupled to a carrier protein.
Synonyms	ILPR; insulin; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin
Reference Data

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