CD53 Mouse Monoclonal Antibody [Clone ID: HD77]

Specifications

Product Data
Clone Name	HD77
Applications	FC, IF, IHC, IP, WB
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising mice with human leukemia cells.
Specificity	This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen. This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD53 molecule
Database Link	Entrez Gene 963 Human UniProt ID P19397
Synonyms	MOX44; tetraspanin-25; Tspan-25; TSPAN25
Reference Data

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