ALDOA Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:1,000 - 1:5,000 ELISA: 1:7,000
Reactivity	Human, Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Aldolase [Rabbit Muscle]
Specificity	Anti-ALDOLASE was prepared from monospecific antiserum by a delipidation, salt fractionation and ion exchange chromatography.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase [Rabbit Muscle].
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 100009055 Rabbit UniProt ID P00883
Background	Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Synonyms	goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase
Note	Anti-Aldolase Antibody has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 µg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 is suggested for this product.  Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Reference Data

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