AAO Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:2,500 - 1:15,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Ascorbate Oxidase [cucurbita]
Specificity	Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].  Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID P24792
Background	Anti-Ascorbate Oxidase antibody is cytoplasmic and performs binding with copper ions. Anti-Ascorbate Oxidase belongs to the multicopper oxidase family and may be a factor in the redox system involving ascorbic acid, acting as a catalyst. Found prevalently in plants, Ascorbate Oxidase has been suggested to increase plant growth. Anti-Ascorbate Oxidase antibody is ideal for researchers interested in the investigation of Cell Biology.
Synonyms	rabbit anti-Ascorbate Oxidase Antibody biotin Conjugation, biotin conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO
Note	Anti-Ascorbate Oxidase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reference Data

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