Monkey IgG (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Monkey IgG (Fc specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP, WB
Recommend Dilution	Can be used: • In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates. • To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. • To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilution: 1/20-1/80.
Reactivity	Monkey
Host	Goat
Immunogen	Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. Label: FITC State: Lyophilised Hyperimmune Ig fraction Label: Fluorescein isothiocyanate isomer 1 Absorption emission: 492nm / 515nm Molar radio: 2.2
Concentration	10.0 mg/ml
Purification	DEAE-column Chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.

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