Human IL-5 ELISA Kit (96-well)

Specifications

Product Data
Description	Human IL-5 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	3.9-250pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IL-5 Ligand
Sensitivity	2pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-5 (8x12) 2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 3 pieces Adhesive Films package insert
Background	Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the .-helical group of cytokines . Unlike other family members, it is present as a covalently linked antiparallel dimer . The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in HodgkinÂ?s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1-3, 6-8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow . The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R.) and a shared Signaltransducing subunit, .c . IL-5 R. first binds IL-5 at low affinity, then associates with preformed .c dimers, forming a high-affinity receptor . IL-5 also binds proteoglycans, potentially enhancing its activity . Soluble forms of IL-5 R. antagonize IL-5 and can be found in vivo . In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1-3, 9, 10, 15). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release .
Gene Symbol	IL5
Standard Curve	Representative standard curve for IL-5 ELISA. IL-5 was diluted in serial two-fold steps in Sample Diluent.

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