Rat IFN gamma ELISA Kit

Specifications

Product Data
Description	Rat IFN gamma ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Rat IFN gamma
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2 pg/ml-2000 pg/ml
Sample Type	Cell culture supernates and serum.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of rat IFN gamma
Sensitivity	<6 pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- rat IFN gamma antibody 1 Lyophilized recombinant rat IFN gamma standard 10 ng/tubex2 Biotinylated anti- rat IFN gamma antibody 100 ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100 ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12 ml ABC diluent buffer 12 ml TMB color developing agent 10 ml TMB stop solution 10 ml Plate seaeler 4 Piece Wash Buffer (25x) 20 ml
Background	Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Gene Symbol	Ifng
Standard Curve	Representative standard curve for Rat IFN gamma PicoKine ELISA Kit

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