Human Thrombomodulin ELISA Kit

CAT#: EA100576

Human Thrombomodulin ELISA Kit



CNY 5,000.00


货期*
3周

规格
    • 1 x 96 wells

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Specifications

Product Data
Description Human Thrombomodulin ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Thrombomodulin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Thrombomodulin
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components 96-well plate precoated with anti- human Thrombomodulin antibody 1
Lyophilized recombinant human Thrombomodulin standard 10ng/tubex2
Biotinylated anti- human Thrombomodulin antibody 130ul(dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
Sample diluent buffer 30 ml
Antibody diluent buffer 12ml
ABC diluent buffer 12ml
TMB color developing agent 10ml
TMB stop solution 10ml
Wash Buffer (25x) 20ml
Plate Sealers 4 pieces
Background Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).
Gene Symbol THBD
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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