Leptin Sandwich ELISA Kit

Specifications

Product Data
Description	Leptin Sandwich ELISA Kit
Size	1 kit
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0 - 100 ng/mL
Sample Type	Cell culture supernatant, serum, plasma
Sample Volume	15 ul
Specificity	Natural and recombinant human Leptin Sandwich
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	The Leptin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on a Leptin molecule. An aliquot of specimen sample containing endogenous Leptin is incubated in the coated well with a specific biotinylated monoclonal anti Leptin antibody. A sandwich complex is formed. After incubation the unbound material is washed off and a Steptavidin Peroxidase Enzyme Complex is added for detection of the bound Leptin. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Leptin in the specimen sample.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.