Mouse Slit2 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of mouse SLIT2 in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse SLIT2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml - 10.000 pg/ml
Specificity	This kit is used for quantitative detection of Mouse SLIT2
Sensitivity	<50 pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- mouse SLIT2 antibody | 1 Lyophilized recombinant mouse SLIT2 standard | 10ng/tube×2 Biotinylated anti- mouse SLIT2 antibody | 100μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4 Wash Buffer (25x) 20 ml
Background	Slit homolog 2 protein is a protein that in humans is encoded by the SLIT2 gene. It is mapped to 5; 5 B3. The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants.
Gene Symbol	Slit2

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