Human IL1 alpha (IL1A) ELISA KIT (96 T)

Specifications

Product Data
Description	Human IL-1sRII ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human IL-1sRII.
Sensitivity	15 pg/ml
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Both IL-1α and IL-1sRII are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. IL-1α and IL-1sRII exert their effects by binding to specific receptors. Two distinct receptors have been identified that bind both forms of IL-1. Common to each is an extracellular immunoglobulin-like (Ig-like) domain that places both IL-1 receptors (IL-1 R) in the type IV cytokine receptor family. The first IL-1 receptor (IL-1 RI) is an 80 kDa transmembrane glycoprotein that has been isolated from astrocytes, chondrocytes, endothelium, fibroblasts, keratinocytes, neurons, oocytes, pancreatic β-cells, smooth muscle cells, and T cells. The second type of IL-1 R (IL-1 RII) is a 60-68 kDa transmembrane glycoprotein which has been found on B cells, keratinocytes, monocytes, neutrophils, and T cells. The two receptors show approximately 28% homology in their extracellular domains but differ significantly in their cytoplasmic regions where IL-1 RI contains 215 amino acid (aa) residues compared to only 29 for IL-1 RII. Depending on species and cell type, the two receptors show differential binding characteristics for IL-1α and IL-1sRII. In general, IL-1α binds better to the type I receptor, and IL-1sRII binds better to the type II receptor.
Gene Symbol	IL1A

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