Human AGRP ELISA KIT (96 T)

Specifications

Product Data
Description	Human AgRP ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human AgRP.
Sensitivity	30 pg/ml
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a secreted orexigenic (appetite-stimulating) neuropeptide with important roles in the regulation of energy homeostasis via its inhibitory actions on the melanocortin system. Human AgRP is 132 amino acids (aa) with eleven cysteine residues, ten of which are in the mature peptide and form five disulfide bonds. Human and murine AgRP are approximately 81% identical at the amino acid level, with the highest identity found in the C-terminal region. AgRP shares sequence and structural homology with Agouti, a protein found in skin that regulates coat color in rodents via inhibition of α-melanocyte-stimulating hormone (α-MSH) on melanocortin receptor 1 (MC-1). In contrast, AgRP is localized mainly to the adrenal gland and a subset of neurons in the hypothalamic arcuate nucleus where it acts as an antagonist of α-MSH on MC-3 and MC-4 receptors. AgRP likely plays a role in human energy homeostasis, and several studies suggest that genetic mutations and altered AgRP levels might be associated with appetite regulation and obesity.
Gene Symbol	AGRP

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