Human MMP2 ELISA KIT (96 T)

Specifications

Product Data
Description	Human MMP-2 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	312.5-20000 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human MMP-2.
Sensitivity	150pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,20000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Human MMP-2 is secreted as a nonglycosylated 72 kDa proenzyme (pro-MMP-2) that contains an 80 amino acid (aa) propeptide and a 551 aa mature region. The propeptide contains a cysteine switch motif that is conserved in MMPs and maintains MMP-2 in a latent state. Removal of the propeptide can be catalyzed by membrane-type MMPs (MT-MMPs) or by the serine proteases Thrombin and activated Protein C. The resulting mature and active enzyme consists of a catalytic domain which is interrupted by three contiguous fibronectin type II-like domains and a C-terminal hemopexin-like domain. Both the pro- and active forms of human MMP-2 share 96-97% aa sequence identity with mouse, rat, porcine, and canine pro-MMP-2. MMP-2 is widely expressed during development and is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. It promotes the progression of tumors to an angiogenic and metastatic phenotype in vivo. MMP-2 is elevated in many body fluids in these environments.
Gene Symbol	MMP2

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