Human TIMP1 ELISA KIT (96 T)

Specifications

Product Data
Description	Human TIMP-1 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human TIMP-1.
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Matrix Metalloproteinases (MMPs) are zinc-dependent endopeptidases that catalyze degradation of extracellular matrix proteins, thereby controlling such processes as development, tissue remodeling, wound healing and tumor metastasis . The activity of MMPs is controlled by regulation of expression and secretion, by proteolytic activation of pro-enzymes and by the Tissue Inhibitors of Metalloproteinases (TIMPs). TIMP-1 is a 184 amino acid residue glycosylated protein, though glycosylation is not necessary for activity . It has 12 cysteines (conserved among all TIMPs) that form disulfide bonds in a pattern that gives distinct N- and C-terminal domains . The N-terminal domain contains sites that bind to the MMP substrate-binding site . Binding of TIMP-1 does not leave a peptide bond in position for proteolysis and is not cleaved . The TIMP/MMP complex can dissociate to yield enzyme and active TIMP-1 . The C-terminal domain binds to an external site on MMPs, increasing overall affinity. TIMP-1 binds with high affinity to the inactive pro-MMP-9, forming a complex in which TIMP-1 retains its ability to inhibit the activity of another active MMP via its N-terminal domain TIMP-1 is widely synthesized by many cells and tissues . Transcription of the TIMP-1 gene is induced by pro-inflammatory cytokines (IL-1, IL-6, OSM, LIF and TNF-α), TGF-β1 and phorbol esters . Many physiological functions of TIMP-1 are closely tied to the functions of MMPs, and an improper balance of MMP and TIMP production correlates with pathological conditions such as arthritis, tumor growth and metastasis . On the other hand, TIMP-1 was independently discovered as an erythroid potentiating activity , an activity that appears to be functionally distinct from MMP inhibitory activity . TIMP-1 binds to certain cell lines and is translocated to the nucleus . It inhibits apoptosis in B cells , further suggesting that it independently functions in multiple ways to support survival and growth of cells in contrast to its function of inhibition of MMPs.
Gene Symbol	TIMP1

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