Mouse Tgfb1 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse TGF-β1 ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse TGF-β1.
Sensitivity	15 pg/ml
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Transforming growth factor beta (TGF-β) proteins (including the three closely related mammalian isoforms TGF-β1, -2 and -3) are pleiotropic cytokines that regulate extracellular matrix production, wound healing, immune functions, cell proliferation and differentiation. They belong to the large TGF-β superfamily, which also includes the activins/inhibins/MIS, bone morphogenetic proteins (BMPs), growth / differentiation factors (GDFs), Lefty1 and 2, and the distantly related GDNF family of neurotrophic factors. All family members show a characteristic cysteine-knot that is formed from multiple intrachain disulfide bonds. The mouse, rat, porcine and canine TGF-β1 cDNAs encode a 390 amino acid (aa) residue precursor that contains a 29 aa signal peptide and a 361 aa pro-protein. The pro-protein for each species is proteolytically processed via a furin-like convertase to generate an N-terminal 249 aa latency-associated peptide (LAP), and a C-terminal 112 aa mature TGF-β1. Both LAP and mature TGF-β1 exist as disulfide-linked homodimers.
Gene Symbol	Tgfb1

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