Mouse Ccl20 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse MIP-3α Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse MIP-3a.
Sensitivity	8pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Mouse MIP-3α, otherwise known as CCL20, LARC, and Exodus, is an 8 kDa protein belonging to the β-, or CC chemokine sub-family. It is synthesized as a 97 amino acid (aa) precursor that contains a 27 aa signal sequence plus a 70 aa mature segment. Mature mouse MIP-3α contains no potential N-linked glycosylation sites. Although human MIP-3α is suggested to contain an O-linked ribose, rodent MIP-3α does not contain any proline rich motifs that might indicate glycosylation. MIP-3α is known to exist in multiple isoforms. An alternate splice form lacking the N-terminal alanine exists in both mouse and human. In addition, human MIP-3α apparently undergoes proteolytic processing at the C-terminus to generate peptides that are shorter by one, three, and six amino acid (aa) residues. All isoforms are equally bioactive. It is possible that mouse MIP-3α may be processed similarly. The mature region of mouse MIP-3α shares 73% and 70% aa sequence identity with mature rat and human MIP-3α, respectively. Among mouse β-chemokines, MIP-3α is distantly related to MIP-3β sharing 34% aa sequence identity. Cells known to express MIP-3α include monocytes, macrophages, microglia and astrocytes, mast cells, neutrophils, keratinocytes, intestinal epithelium, osteoblasts, fibroblasts, and endothelial cells.
Gene Symbol	Ccl20

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