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Wnt3 (NM_009521) Mouse Untagged Clone
CAT＃: MC209651
Wnt3 (untagged) - Mouse wingless-related MMTV integration site 3 (Wnt3), (10ug)
CNY 4,024.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Int-4; Wnt-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209651 representing NM_009521
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCACCTGCTCGGGCTGCTACTCGGCCTCCTGCTCAGTGGCACCAGGGTCCTCGCTGGCTACC CAATTTGGTGGTCCCTGGCCCTGGGCCAGCAGTACACATCTCTGGCCTCCCAGCCTCTGCTCTGCGGCTC CATCCCAGGCCTGGTCCCCAAGCAACTGCGCTTCTGCCGCAATTACATCGAGATCATGCCCAGCGTAGCA GAAGGTGTGAAGCTGGGCATCCAGGAGTGCCAGCATCAGTTCCGGGGCCGCCGGTGGAACTGTACCACCA TAGATGACAGCCTGGCCATCTTTGGGCCTGTCTTGGACAAAGCCACCCGTGAATCGGCCTTCGTGCATGC CATCGCCTCGGCTGGTGTCGCCTTCGCAGTCACACGCTCCTGCGCTGAGGGAACCTCCACCATCTGCGGC TGTGACTCACATCATAAGGGGCCACCTGGAGAAGGCTGGAAGTGGGGCGGCTGCAGCGAGGACGCCGACT TCGGGGTGCTGGTGTCCCGGGAATTTGCGGATGCGCGGGAGAACAGGCCAGATGCCCGCTCAGCTATGAA CAAGCACAACAATGAAGCAGGCCGAACGACCATCCTGGACCACATGCACCTAAAGTGTAAATGCCACGGG TTGTCCGGCAGCTGCGAGGTGAAGACCTGCTGGTGGGCCCAGCCCGACTTCCGTGCCATTGGCGACTTCC TCAAGGACAAGTACGACAGTGCCTCCGAGATGGTGGTGGAGAAACACCGTGAGTCCCGAGGCTGGGTGGA GACCCTGCGGGCTAAGTACGCGCTCTTCAAGCCACCCACCGAGAGGGACCTGGTCTACTACGAGAACTCC CCCAACTTTTGTGAGCCCAACCCAGAGACGGGCTCCTTTGGTACCAGGGACCGGACTTGCAATGTCACCT CCCACGGCATCGATGGCTGCGATCTGCTGTGCTGTGGCCGGGGCCACAACACGAGGACGGAGAAACGGAA GGAGAAATGCCATTGCGTCTTCCACTGGTGCTGCTATGTCAGCTGCCAAGAGTGTATTCGCATCTACGAT GTGCACACCTGCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009521 |
| Insert Size | 1068 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009521.2, NP_033547.1 |
| RefSeq Size | 3115 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 22415 |
| UniProt ID | P17553 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (By similarity). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PubMed:10431240). Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development (PubMed:12569130).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222492 | Wnt3 (tGFP-tagged) - Mouse wingless-related MMTV integration site 3 (Wnt3), (10ug) |
CNY 7,088.00 |
|
| MR222492 | Wnt3 (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3 (Wnt3) |
CNY 5,488.00 |
|
| MR222492L3 | Lenti ORF clone of Wnt3 (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3 (Wnt3) |
CNY 5,890.00 |
|
| MR222492L4 | Lenti ORF clone of Wnt3 (mGFP-tagged) - Mouse wingless-related MMTV integration site 3 (Wnt3) |
CNY 5,890.00 |
