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Cntf (NM_170786) Mouse Tagged ORF Clone
CAT＃: MG201984
- TrueORF®
Cntf (tGFP-tagged) - Mouse ciliary neurotrophic factor (Cntf), transcript variant 1
ORF Plasmid: DDK
"NM_170786" in other vectors (3)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI429687 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201984 representing NM_170786
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCGCAGAGCAATCACCTCTGACCCTTCACCGCCGGGACCTCTGTAGCCGCTCTATCTGGCTAG CAAGGAAGATTCGTTCAGACCTGACTGCTCTTATGGAATCTTATGTAAAACATCAAGGCCTGAATAAAAA TATCAGCCTTGACTCAGTGGATGGTGTACCAGTGGCAAGCACTGATCGCTGGAGTGAGATGACTGAGGCA GAGCGACTCCAAGAGAACCTCCAGGCTTACCGTACCTTCCAAGGGATGTTAACCAAGCTTTTAGAAGACC AGAGAGTGCATTTCACCCCGACTGAAGGTGACTTCCATCAGGCAATACATACTCTTACGCTCCAAGTTTC TGCCTTCGCCTACCAGCTAGAGGAGTTAATGGCGCTTCTGGAACAGAAGGTCCCTGAAAAAGAGGCTGAT GGGATGCCTGTCACAATTGGAGATGGTGGCCTCTTTGAGAAGAAGCTGTGGGGCTTGAAGGTCCTTCAAG AGCTTTCACAGTGGACTGTGAGGTCTATCCATGACCTCCGTGTCATTTCTTCTCATCACATGGGAATCTC AGCACATGAGAGCCATTATGGGGCCAAGCAAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201984 representing NM_170786
Red=Cloning site Green=Tags(s) MAFAEQSPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNISLDSVDGVPVASTDRWSEMTEA ERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLTLQVSAFAYQLEELMALLEQKVPEKEAD GMPVTIGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHHMGISAHESHYGAKQM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_170786 |
| ORF Size | 594 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170786.2, NP_740756.1 |
| RefSeq Size | 1205 bp |
| RefSeq ORF | 597 bp |
| Locus ID | 12803 |
| UniProt ID | P51642 |
| Gene Summary | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR201984 | Cntf (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor (Cntf) |
CNY 2,400.00 |
|
| MR201984L3 | Lenti ORF clone of Cntf (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor (Cntf) |
CNY 4,750.00 |
|
| MR201984L4 | Lenti ORF clone of Cntf (mGFP-tagged) - Mouse ciliary neurotrophic factor (Cntf) |
CNY 4,750.00 |
