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Tgif1 (NM_009372) Mouse Tagged ORF Clone
CAT＃: MG203642
- TrueORF®
Tgif1 (tGFP-tagged) - Mouse TG interacting factor 1 (Tgif1)
ORF Plasmid: DDK
"NM_009372" in other vectors (4)
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CNY 3,040.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA959811; AI462167; Tgif |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203642 representing NM_009372
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGCAAGAAGGGTCTTGTTGCAGCATCTGGCAGTGACTCTGAGGATGAAGACAGCATGGACAGTC CCCTGGACCTTTCCTCATCAGCAGCCTCTGGCAAGAGAAGGAGGAGAGGCAATCTGCCCAAGGAGTCAGT CCAGATTCTGCGAGACTGGCTGTATGAACACAGATACAACGCCTATCCCTCAGAGCAAGAGAAAGCACTG CTGTCCCAGCAGACACACCTGTCCACACTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGCAGGCTCC TTCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACGATTTCCCGCCGTGGGGCCAAGAT TTCAGAAGCTAGCTCTATTGAAGCTGCAATGGGTATCAAAAACTTCATGCCAACTCTAGAAGAGAGCCCA TTTCATTCCTGCGTAGTTGGACCCAACCCAACCCTAGGGAGACCAGTGTCTCCCAAACCTCCCTCCCCAG GATCCATTTTGGCTCGCCCGTCAGTGATCTGCCATACCACTGTGACTGCATTGAAGGATGGGCCTTTCTC TCTCTGTCAGCCGATTGGTGTGGGACAGAGTACAGATGTACCGCAAATAGCACCCAGCAACTTTACAGAC ACCTCTCTCGTGTACCCAGAGGACACTTGCAAATCTGGACCCAGTCCAAACCCTCAGAGTGGTCTTTTCA ACACTCCTCCCCCTACTCCACCAGACCTCAACCAGGATTTTAGTGGATTCCAGCTTCTAGTGGATGTTGC ACTCAAACGAGCGGCAGAGATGGAGCTTCAGGCCAAACTCACAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203642 representing NM_009372
Red=Cloning site Green=Tags(s) MKSKKGLVAASGSDSEDEDSMDSPLDLSSSAASGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKAL LSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISEASSIEAAMGIKNFMPTLEESP FHSCVVGPNPTLGRPVSPKPPSPGSILARPSVICHTTVTALKDGPFSLCQPIGVGQSTDVPQIAPSNFTD TSLVYPEDTCKSGPSPNPQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009372 |
| ORF Size | 816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009372.3, NP_033398.2 |
| RefSeq Size | 1634 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 21815 |
| UniProt ID | P70284 |
| Gene Summary | Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Negative Interplay of Retinoic Acid and TGF-ß Signaling Mediated by TG-Interacting Factor to Modulate Mouse Embryonic Palate Mesenchymal-Cell Proliferation
,Liu, X;Zhang, H;Gao, L;Yin, Y;Pan, X;Li, Z;Li, N;Li, H;Yu, Z;,
Birth Defects Res. B Dev. Reprod. Toxicol.
,PubMed ID 25477235
[TGIF1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205613 | Tgif1 (untagged) - Mouse TGFB-induced factor homeobox 1 (Tgif1), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MR203642 | Tgif1 (Myc-DDK-tagged) - Mouse TGFB-induced factor homeobox 1 (Tgif1), transcript variant 2 |
CNY 2,760.00 |
|
| MR203642L3 | Lenti ORF clone of Tgif1 (Myc-DDK-tagged) - Mouse TGFB-induced factor homeobox 1 (Tgif1), transcript variant 2 |
CNY 4,660.00 |
|
| MR203642L4 | Lenti ORF clone of Tgif1 (mGFP-tagged) - Mouse TGFB-induced factor homeobox 1 (Tgif1), transcript variant 2 |
CNY 4,660.00 |
